Mouse TNF alpha PicoKine ELISA Kit
- 【品牌】: 博士德
- 【產(chǎn)品貨號(hào)】: EK0527
- 【價(jià)格】: 1200 元
- 【規(guī)格】: 48T/96T
- 【貨期】: 現(xiàn)貨
| 產(chǎn)品說明 | |
| 【品牌】: | 博士德 |
| 【產(chǎn)品貨號(hào)】: | EK0527 |
| 【價(jià)格】: | 1200 元 |
| 【規(guī)格】: | 48T/96T |
| 【貨期】: | 現(xiàn)貨 |
| 【儲(chǔ)存溫度】: | -20 |
Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
典型操作程序：
1 準(zhǔn)備試劑和標(biāo)準(zhǔn)品
2 每孔添加100ul樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)90分鐘，不洗。
3 每孔添加100ul生物素標(biāo)記抗體，37℃反應(yīng)60分鐘。
4 0.01M TBS洗滌3次。
5 每孔添加100ulABC，37℃反應(yīng)30分鐘。
6 0.01M TBS洗滌5次。
7 TMB37℃避光反應(yīng)30分鐘以內(nèi)。
8 加入TMB終止液，讀數(shù)。
